Table 1. Primer sequences and melting temperatures.

Designation	Sequence	Tm
Series A and B
Limiting primers
CF403 S18	GATTATGCCTGGCACCAT	51
CF402 S19	GGATTATGCCTGGCACCAT	54
CF401 S21	CTGGATTATGCCTGGCACCAT	57
CF399 S22	CCTGGATTATGCCTGGCACCAT	59
CF398 S23	TCCTGGATTATGCCTGGCACCAT	61
CF396 S25	TTTCCTGGATTATGCCTGGCACCAT	61
Excess primer
CF479 A20	TGATGACGCTTCTGTATCTA	54
Series C
Limiting primers
CF402 S19t	TGATTATGCCTGGCACCAT	53
CF401 S20	TGGATTATGCCTGGCACCAT	56
CF399 S22	CCTGGATTATGCCTGGCACCAT	59
CF392 S29	CAGTTTTCCTGGATTATGCCTGGCACCAT	64
Excess primers
CF475 A16	GACGCTTCTGTATCTA	47
CF477 A18	ATGACGCTTCTGTATCTA	50
CF479 A20	TGATGACGCTTCTGTATCTA	54
CF483 A24	GCTTTGATGACGCTTCTGTATCTA	59

Sequences and 5′ nucleotide designation are from GenBank accession no. M55115. S, sense strand; A, antisense strand. T_m are based on concentrations of 50 nM for limiting primers and 1,000 nM for excess primers. Primers CF396 S25 and CP398 S23 gave similar results, which are combined in Table 2.