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. 2024 Oct 18;25(20):11200. doi: 10.3390/ijms252011200

Figure 2.

Figure 2

Effect of GHRH-R antagonist MIA-690 (0.1 μM) and Gefitinib (200 μM) on (A) cell adhesion to collagen in PC-3 cells. Results are expressed as percentage of control value at 0 and 60 min. Data correspond to mean ± S.E.M. of at least three independent experiments in triplicate; ***, p < 0.001 vs. control; ###, p < 0.001 vs. treatment with MIA-690 alone; +++, p < 0.001 vs. treatment with Gefitinib alone. (B) β-catenin levels in PC-3 cells by means of Western blot assay with specific antibody. A representative image is shown of at least two independent experiments. (C) Secretion of metalloproteinases (MMPs) 2 and 9 from PC-3 cells by means of Zimography assay. Both gelatinases are detected in their active (MMP-9 and MMP-2) and latent (pro-MMP-9 and pro-MMP-2) forms. The image of at least two independent experiments is shown.