Table 2. The change in the total fluorescence intensities of B4F upon addition of excess SCD, C2 dimer, C2 tetramer, and E2 tetramer.
Protein | Total intensity at 0.18 nM protein (monomer) | Total intensity at 0.25 nM protein (monomer) |
---|---|---|
SCD | 259 | 198 |
C2D | 317 | 272 |
C2T | 260 | 208 |
E2T | 325 | 250 |
No protein | 425 | 425 |
The E2 dimer was not tested with 0.18 and 0.25 nM, but with 1 nM monomer (total intensity = 290); it also showed quenching.