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. 2005 Jun 3;102(24):8567–8572. doi: 10.1073/pnas.0503221102

Fig. 3.

Fig. 3.

Tubacin inhibits binding of HDAC6 with dynein, and when combined with bortezomib, it induces significant accumulation of polyubiquitinated proteins. (A) Hypothetical rationale whereby tubacin enhances cytotoxicity induced by bortezomib [adapted from Kawaguchi et al. (17)]. (B) MM.1S cells were cultured with tubacin (2.5 and 5 μM) for 8 h. Whole-cell lysates were immunoprecipitated with anti-Ub Ab. Immununoprecipitates were subjected to Western blotting with anti-HDAC6 Ab. (C) MM.1S cells were cultured with tubacin (2.5 and 5 μM) for 8 h. Whole-cell lysates were immunoprecipitated with anti-dynein Ab. Immununoprecipitates were then subjected to Western blotting with anti-HDAC6 and dynein Abs. (D) MM.1S and RPMI8226 cells were cultured with tubacin (2.5 and 5 μM) for 24 h. Whole-cell lysates were subjected to Western blot with anti-Ub Ab. (E) MM.1S and RPMI8226 cells were cultured with tubacin (T: 5 μM) and/or bortezomib (B: 5 nM) for 12 h. Whole-cell lysates were subjected to Western blotting with anti-Ub Ab.