Fig. 5.

Tubacin synergistically enhances bortezomib-induced cytotoxicity in patient MM cells without cytotoxicity to PBMCs. (A–D) BMPCs (A–C) and PBMCs (D) from three MM patients were cultured in the presence or absence of tubacin (5 μM) in control media (white bars) and with 10 (gray bars) or 20 nM (black bars) bortezomib for 24 h; cytotoxicity was assessed by MTT assay. (E) MM patient PBMCs were cultured in the presence or absence of tubacin (5 μM). Whole-cell lysates were subjected to Western blotting with anti-HDAC6, Ac-Lys, or α-tubulin Abs.