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. 2001 Sep;75(18):8400–8406. doi: 10.1128/JVI.75.18.8400-8406.2001

FIG. 7.

FIG. 7

Primer extension analysis of HBV RNA expressed from the HBV DNA constructs carrying the Sp1-3 mutation. (A) Primer extension analysis of the C gene transcripts. The locations of the precore RNA, the core RNA, and the hGH RNA are marked. (B) Primer extension analysis of the S gene transcripts. The locations of the pre-S-2 RNA, the major S RNA, and the hGH RNA are marked. Lanes 1, Huh7 cells transfected by the wild-type HBV DNA; lanes 2, Huh7 cells transfected by the HBV DNA genome carrying the Sp1-3 mutation; lanes 3, Huh7 cells transfected by the HBV DNA genome carrying the mutations of all three Sp1 sites.