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(A) pUSE1−CMVT(gagpol) DNA construct. (B) pUSE1−CMVT(envA) DNA construct. ∗, 5′ donor site from the first intron of the human beta-globin gene and the branch and 3′-acceptor of an immunoglobulin gene heavy-chain variable region. AMP, ampicillin resistance gene. The pUSE1− plasmid is a mammalian expression vector derived from the vector pCI-neo (Promega).
