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. 2024 Oct 3;14(10):1261. doi: 10.3390/life14101261

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Detection of virulence genes stx1, stx2, and eae by multiple PCR in E. coli isolates in pig carcasses from slaughterhouse B in two gels. (1) 100 bp marker, (2) negative control, (3) positive control for stx1 gene (388 bp) in E. coli, (4) isolate 10D—negative, (5) isolate 10E—positive for eae gene (570 bp), (6) isolate 11A—positive for eae (570 bp) and stx2 (807 bp) genes, (7) isolate 11D—positive for eae (570 bp), stx1 (388 bp) and stx2 (807 bp) genes, (8) isolate 12A—positive for eae genes (570 bp) and stx1 (388 bp), (9) isolate 12D—positive for eae (570 bp) and stx1 (388 bp) genes.