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. 2024 Oct 3;14(10):1261. doi: 10.3390/life14101261

Table 1.

List of primers used to detect virulence genes in isolates of E. coli.

Gene Sequence of Nucleotides (5′-3′) Size (pb) Annealing Temperature (°C) Reference
Stx-1 F-AGA GCG ATG TTA CGG TTT G
R-TTG CCC CCA GAG TGG ATG
388 50 [35]
Stx-2 F-TGG GTT TTT CTT CGG TAT C
R-GAC ATT CTG GTT GAC TCT CTT
807 50 [35]
eae F-AGG CTT CGT CAC AGT TG
R-CCA TCG TCA CCA GAG GA
570 50 [35]