Table 1.
Rates of ATP hydrolysis (vH) and ATP synthesis (vS) of liposome-bound F0F1 at 23°C, measured at different states during the preparation procedure
| Enzymes |
vH (s−1) |
vS (s−1) |
||
|---|---|---|---|---|
| +DCCD | +DCCD | |||
| F0F1 ‘wild type' | 91±2 | ND | 87±11 | ND |
| F0-bQ64C-F1 | 71±4 | ND | 60±1 | ND |
| F0-bQ64C-Cy5bis-F1 | 75±9 | ND | 48±1 | ND |
| F0-bQ64C (after removal of F1) | 0 | ND | 0 | ND |
| F0-bQ64C-Cy5bis (after removal of F1) | 0 | ND | 0 | ND |
| F0-bQ64C-F1, after removal of F1 and reassembling with F1-ɛH56C | 55±8 | 4±2 | 22±3 | 4±3 |
| F0-bQ64C-F1, after removal of F1 and reassembling with F1-ɛH56C-TMR | 55±9 | 5±1 | 21±3 | 4±2 |
| F0-bQ64C-Cy5bis after removal of F1 and reassembling with F1-ɛH56C | 52±8 | 5±1 | 23±3 | 5±2 |
| F0-bQ64C-Cy5bis after removal of F1 and reassembling with F1-ɛH56C-TMR | 57±19 | 5±2 | 21±4 | 4±0 |
| ND: not determined. | ||||