FIG. 6.

In vitro neutralization assay of HIV-1. Neutralizing activities of sera collected at day 42 from mice immunized intranasally with PVX-2F5E (M1, M4) or WT PVX (M7) and at day 17 from hu-PBL-SCID mice immunized with PVX-2F5E-pulsed (H4) or WT PVX-pulsed (H6) DCs were determined by syncytium inhibition assay. Percentages of neutralization, evaluated as described in Materials and Methods, are plotted against serum dilution. Each line shows the neutralizing activity of a representative serum from one mouse in each group. Similar results were obtained using serum from the other mice in the same group.