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. 2005 May 26;24(12):2183–2193. doi: 10.1038/sj.emboj.7600691

Figure 6.

Figure 6

Editing normally occurs in muscle and toxic levels of ADAR activity cause hyperediting of specific editing sites in a muscle transcript. (A) Editing in the Ca-alpha 1D transcript encoding an L-type voltage-gated calcium channel normally occurs in muscle. Percentage editing at five sites in the Ca-alpha 1D transcript based on multiple sequence chromatograms of several RT–PCR product pools from RNA isolated from head (neuron), dorsal thorax (muscle) or whole body of adult Canton S flies is shown. The editing sites are named by the position of the altered base and the codon change introduced. Locations of residues changed by editing in this channel are indicated on the generic calcium channel structure in Figure 3. (B) Hyperediting of sites in the Ca-alpha 1D transcript in embryos and L1 larvae expressing either no additional ADAR or ADAR 3/4 S/G or ADAR 3/4 S isoforms under the control of the muscle-specific Mef2-GAL4 driver. Editing was analysed by bulk sequencing RT–PCR products derived from RNA isolated from 48 h collections of embryos and larvae.