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Detection of single phosphorylation mutants with mutations at residues 151 and 153 in 293 T cells. Transfection, immunoprecipitation, and SDS-PAGE analysis were performed as described in the legend to Fig. 1. The various Rex mutants analyzed are indicated. The data suggest that serine 151 is more important than serine 153 for the phosphorylation-induced mobility shift from p24rex to p26rex.
