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. 2024 Sep 27;12(10):1951. doi: 10.3390/microorganisms12101951

Figure 3.

Figure 3

Effects of irrigation solutions at low doses on neutrophil plasma membrane damage. Flow cytometric analysis of neutrophil membrane integrity. Neutrophils were incubated in 10% irrigation solutions. Representative plots showing forward (FSC-A) and side scatter (SSC-A) or membrane permeability as measured by increase in Pac Blue staining (dot and histogram plots) of (A) HBSS only, (B) XP, (C) Irrisept, or (D) Betadine. (E) Quantification of neutrophil plasma membrane damage following incubation with irrigation solutions. Data displayed are from three biological replicates. ** p < 0.005, *** p < 0.0005, **** p < 0.0001 as determined by two-way ANOVA followed by Tukey’s multiple comparisons test. Error bars indicate mean ± SD.