TABLE 1.

Cross-neutralization activity in serum from infected or DNA-vaccinated hamsters

Hamster serum sourcea	PRNT80 or PRNT50d in animals infected with:
	SEOV		HTNV		DOBV		PUUV
	80%	50%	80%	50%	80%	50%	80%	50%
Infectedb with:
SEOV	640	5,120	∗	80	∗	∗	∗	∗
HTNV	∗	160	1,280	5,120	40	320	∗	20
DOBV	20	320	20	160	2,560	10,240	∗	40
PUUV	∗	∗	∗	∗	∗	20	5,120	20,480
Vaccinatedc with:
pWRG/SEO-M	5,120	10,240	320	320	160	320	∗	∗
pWRG/HTN-M	40	80	5,120	5,120	160	640	∗	∗

^aA pool of sera from three to four hamsters with a homologous titer of at least 160 was used. Serum was collected 4 weeks after infection or 3 weeks after the final DNA vaccination. 

^bHamsters were injected i.m. with 2,000 PFU of the indicated virus. 

^cHamsters were gene gun-vaccinated three times at 3-week intervals with the indicated DNA vaccine plasmid. 

^dPRNT values are the reciprocal serum dilutions that neutralize the indicated virus plaque number by 80% or 50%. Homologous titers are shown in boldface. ∗, titer of <20.