TABLE 2.
Cross-neutralization activity in serum from vaccinated monkeys
Monkey serum sourcea | Monkeye | PRNT80 or PRNT50d in animals infected with:
|
|||||||
---|---|---|---|---|---|---|---|---|---|
SEOV
|
HTNV
|
DOBV
|
PUUV
|
||||||
80% | 50% | 80% | 50% | 80% | 50% | 80% | 50% | ||
Vaccinatedb with pWRG/SEO-M(x) | CH03 | 640 | 1,280 | ∗ | ∗ | 40 | 160 | ∗ | ∗ |
CH32 | 1,280 | 1,280 | ∗ | 80 | 320 | 640 | ∗ | 40 | |
Vaccinatedb with pWRG/HTN-M(x) | CH28 | ∗ | ∗ | 2,560 | 2,560 | ∗ | 320 | ∗ | ∗ |
CH02 | ∗ | ∗ | 5,120 | 10,240 | 160 | 320 | ∗ | ∗ | |
CH27 | ∗ | 20 | 640 | 1,280 | 80 | 320 | ∗ | ∗ | |
Vaccinatedc with rVV/HTN-M+S | 62F | ∗ | ∗ | 40 | 160 | ∗ | 80 | ∗ | ∗ |
CH21 | ∗ | ∗ | 320 | 640 | ∗ | 160 | ∗ | ∗ | |
AA016 | ∗ | ∗ | 320 | 320 | ∗ | 20 | ∗ | ∗ |
Monkey serum was collected 3 weeks after the final vaccination.
Monkeys were gene gun-vaccinated three times at 3-week intervals with the indicated DNA vaccine plasmid.
Monkeys were injected subcutaneously with recombinant vaccinia virus vaccine two times at a 42-day interval.
PRNT values are the reciprocal serum dilutions that neutralize the indicated virus plaque number by 80% or 50%. Homologous titers are shown in boldface. ∗, Titer of <20.
Monkey identification code.