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. 2024 Sep 30;12(10):1999. doi: 10.3390/microorganisms12101999

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Tissue response after transformation with different Agrobacterium strains carrying pCTAGV-KCN3. (A) EHA105 (mix of proliferating and brown necrotic tissues). (B) Wild-type 1D1416 (healthy and proliferating tissues). (C) Wild-type 1D1104 (bacterial overgrowth, necrotic and dying tissues). (D) The effect of surfactant (SF) on the mortality rate of transformed Carrizo tissue with different Agrobacterium strains. Three replicates were preformed; each replicate contained at least 30 explants. Statistics were performed on the average of the three technical replicates. Significant difference (*) between SF-treated and non-treated samples (p < 0.05, Student’s t-test). See Supplementary Table S2 for data set.