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Shoot regeneration from Carrizo epicotyls transformed with 1D1416/pCTAGV-KCN3. (A) Abnormal shoot bud regenerated from tissue without gall formation, no surfactant (SF) added. (B,C) Normal regenerated transgenic shoots from epicotyl tissues treated with SF on SMM (upper images are from a light microscope and bottom images are captured using a DsRed fluorescent filter). (D) Carrizo shoots isolated from the edge of gall tissue. (E) Isolated Carrizo shoots in rooting medium.
