FIG. 2.

Replication of a subgenomic replicon derived from an infectious HCV1b clone. (A) Huh7 cells were electroporated with RNA from HCV1bneo, and colonies were stained 16 days after the transfection. (B) Northern blot analysis of total RNA isolated from pooled cells transfected with the indicated RNAs. Genomic HCV RNA (vRNA) was detected as described for Fig. 1. (C) In vitro translation of viral RNA transcribed from plasmids I₃₇₇/NS3-3′, HCV1bneo, HCVNneo, D2Rneo, and D2Ineo (lanes 1 to 5) . Luciferase was translated from pLuc as a positive control (lane 6). The positions of HCV NS3 and the neomycin phosphotransferase (neo) are indicated.