Figure 5.

The evolutionary history of the WSP gene was inferred by using the maximum likelihood method and Tamura–Nei model and shows the name of the host where Wolbachia were detected. The tree with the highest log likelihood (−5397.55) is shown. Bootstrap values are shown next to the branches. Initial tree(s) for the heuristic search were obtained automatically by applying the Neighbor Joining and BioNJ algorithms to a matrix of pairwise distances estimated using the maximum composite likelihood (MCL) approach and then selecting the topology with the superior log likelihood value. The tree is drawn to scale, with the branch lengths measured in the number of substitutions per site. This analysis involved 75 nucleotide sequences. The codon positions included were 1st + 2nd + 3rd + noncoding. There are 688 positions in the final dataset. The evolutionary analyses were conducted in MEGA X. The blue labels show the sequences retrieved from the NCBI database.