Table A1.

List of primers used in this study.

Name	Primers	Pb	Tm	Reference
Wol_F Wol_R Probe	Forward: 5′-CCAGCAGCCGCGGTAAT-3′ Reverse: 5′-CGCCCTTTACGCCCAAT-3′ Probe: 5′-CGGAGAGGGCTAGCGTTATTCGGAATT-3′	464	60	(Mee et al., 2015) [42]
WSP	Forward: 5′-GTCCAATARSTGATGARGAAAC-3′ Reverse: 5′-CYGCACCAAYAGYRCTRTAAA-3′	540	51	(Baldo et al., 2006) [42]
WolbF Wspec	Forward: 5′-GAAGATAATGACGGTACTCAC-3′ Reverse: 5′-AGCTTCGAGTGAAACCAATTC-3′	800	45	(Carvajal et al., 2019) [44]
BC1culicm JeR2m	Forward: 5′-CAGGAAACAGCTATGACCCAAARAATCARAAYARRTGTTG-3′ Reverse: 5′-GTAAAACGACGGCCAGTTCWACWAAYCAYAAARWTATTG-3′	658	51	(Hebert et al., 2003) [39]
CoxA	Forward: 5′-TTGGRGCRATYAACTTTATAG-3′ Reverse: 5′-CTAAAGACTTTKACRCCAGT-3′	487	53	(Baldo et al., 2006) [42]
GatB	Forward: 5′-GAKTTAAAYCGYGCAGGBGTT-3′ Reverse: 5′-TGGYAAYTCRGGYAAAGATGA-3′	471	54	(Baldo et al., 2006) [42]
HcpA	Forward: 5′-GAAATARCAGTTGCTGCAAA-3′ Reverse: 5′-GAAAGTYRAGCAAGYTCT G-3′	515	48	(Baldo et al., 2006) [42]
FbpA	Forward: 5′-GCTGCTCCRCTTGGYWTGAT-3′ Reverse: 5′-CCRCCAGARAAAAYYACTATT C-3′	509	53	(Baldo et al., 2006) [42]
FtsZ	Forward: 5′-TACTGACTGTTGGAGTTGTAACTAAGCCGT-3′ Reverse: 5′-TGCCAGTTGCAAGAACAGAAACTCTAACTC-3′	600	58	(Hu et al., 2020) [46]