Table A2.

Recommended PCR cycles for HOT FirePol DNA Polymerase.

Cycle step	Temp.	Time	Cycles
Initial denaturation	95 °C	15 min	1
Denaturation	95 °C	30–60 s	26–35
Annealing	50–68 °C	30–60 s
Elongation	72 °C	1–4 min
Final elongation	72 °C	10 min	1