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Recommended qPCR cycles for SuperScript™ III Platinum™ One-Step qRT-PCR Kit.
| Cycle Step | Temp. | Time | Cycle |
|---|---|---|---|
| Activate uracile_N_glycosylase | 50 °C | 2 min | 1 |
| Activation Taq | 95 °C | 10 min | 1 |
| Denaturation | 95 °C | 15 s | 40 |
| Hybridization–polymerization | 60 °C | 1 min |
