Figure 1.

Experimental protocols used in the current studies. In acute experiments on cardiac tissues collected from Wistar rats, after 30-min incubation with 1 µM cannabidiol (CBD) or its vehicle, in accordance with Protocol 1, tissues were exposed to 30 min hypoxia and 30 min reoxygenation with followed increasing concentrations of the nonselective β-adrenoceptor agonist isoprenaline (ISO) administration (0.01 nM–10 µM). In Protocol 2, after incubation with CBD/vehicle tissues were exposed to ISO (0.01 nM–10 µM) and then to H/R. In chronic experiments, left atria isolated from spontaneously hypertensive rats (SHR) and their normotensive control (WKY) rat 24 h after 2-week treatment with CBD 10 mg/kg/day or its vehicle were subjected to H/R with following ISO administration (0.01 nM–30 µM, Protocol 3).