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. 2024 Oct 14;29(20):4864. doi: 10.3390/molecules29204864

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The PchEHA gene mutation library constructed via random mutation. (A) Electrophoresis analysis of mutant PCR products; dNTP/dITP in A1 lane is 25/175, dNTP/dITP in A2 lane is 50/150, dNTP/dITP in A3 lane is 75/125, and dNTP/dITP in A4 lane is 200/0; (B) electrophoresis analysis of PCR products without primer assembly, and DNA fragments obtained by EndoV enzymatic digestion serve as mutual primers in assembly PCR to enhance the mutational diversity of the DNA fragments. M: λ-EcoT14 I digest DNA Marker.