Table 1.

List of primers for mRNA expression.

Gene	Forward	Reverse
Claudin 3	5′-CCC AGC CTA CGG AGT TAC CC-3′	5′-TGC CGA TGA ATG CCG AAA CG-3′
GFAP	5′-AAT CTC ACA CAG GAC CTC GGC-3′	5′-AGC CAA GGT GGC TTC ATC CG-3′
CD11b	5′-TCC AAC CTG CTG AGG AAG CC-3′	5′-TCG ATC GTG TTG ATG CTA CCG-3′
IBA1	5′-GAG CTA TGA GCC AGA GCA AGG ATT T-3′	5′-ACT CCA TGT ACT TCG TCT TGA AGG-3′
TLR4	5′-TTG CAT CTG GCT GGG ACT CTG-3′	5′-TTC AGG GGG TTG AAG CTC AGA T-3′
TLR2	5′-AAG AGC ATC GGC TGG AGG TC-3′	5′-TGG AGC TGC CAT CAC ACA CA-3′
TNFα	5′-GAA CTC CAG GCG GTG TCT GT-3′	5′-CTG AGT GTG AGG GTC TGG GC-3′
NFκB	5′-CCT CCA CCC CGA CGT ATT GC-3′	5′-GCC AAG GCC TGG TTT GAG AT-3′
IL1β	5′-ATG TCT TGC CCG TGG AGC TT-3′	5′-ATG GGT CAG ACA GCA CGA GG-3′
TFAM	5′-GCT TCC AGG GGG CTA AGG ATG-3′	5′-TCG CCC AAC TTC AGC CAT TT-3′
PGC1α	5′-CAG GAG CTG GAT GGC TTG GG-3′	5′-GGG CAA AGA GGC TGG TCC T-3′
FIS1	5′-CTG CGG TGC AGG ATG AAA GAC-3′	5′-GGC GTA TTC AAA CTG CGT GCT-3′
DRP1	5′-ACA ACA GGA GAA GAA AAT GGA GTT G-3′	5′-AGA TGG ATT GGC TCA GGG CT-3′
MFN2	5′-TCC TGA ACA ACC GCT GGG AT-3′	5′-GAT CCA CCA CGC CTA GCT CA -3′
MFN1	5′-AGC TCG CTG TCA TTG GGG AG-3′	5′-TCC CTC CAC ACT CAG GAA GC-3′
Complex III	5′-GCA GTC CTC GCA TCC TAC CT-3′	5′-CTC CCG AGT GCT GTA GGC AT-3′
Complex I	5′-GGT TTG TCT ACA TCG GCT TCC-3′	5′-TAC AGA AGC TGG CGA TGC AAA-3′
PINK1	5′-TCG GCC TGT CAG GAG ATC CA-3′	5′-CAT TGC AGC CCT TGC CGA TG-3′
β-actin	5′-ACA ACC TTC TTG CAG CTC CTC C-3′	5′-TGA CCC ATA CCC ACC ATC ACA-3′

Abbreviations: GFAP, glial fibrillary acidic protein; CD11b, cluster of differentiation molecule 11b; IBA1, ionized calcium binding adaptor molecule 1; TLR4, toll-like receptor 4; TLR2, toll-like receptor 2; TNFα, tumor necrosis factor-α; NFκB, nuclear factor kappa-light-chain enhancer of activated B cells; IL1β, interleukin 1β; TFAM, mitochondrial transcription factor A; PGC1α, peroxisome proliferative activated receptor alpha; FIS1, fission 1; DRP1, dynamin-related protein 1; MFN2, mitofusin 2; MFN1, mitofusin 1; PINK1, PTEN-induced kinase 1.