Table 2.
Summary of antibody dilutions and conditions used in Western blot analysis.
| Primary Antibody | Species | Dilution | Vendor | Secondary Antibody | Dilution | Vendor |
|---|---|---|---|---|---|---|
| Claudin 3 | Rabbit polyclonal | 1:1000 | Invitrogen Thermo Fisher Scientific, Waltham, MA, USA |
Goat anti-rabbit HRP | 1:2000 | Cell Signaling Technology, Inc., Danvers, MA, USA |
| GFAP | Mouse monoclonal | 1:2000 | Invitrogen Thermo Fisher Scientific, Waltham, MA, USA |
Horse anti-mouse HRP | 1:2000 | Cell Signaling Technology, Inc., Danvers, MA, USA |
| IBA1 | Rabbit monoclonal | 1:1000 | Cell Signaling Technology, Inc., Danvers, MA, USA |
Goat anti-rabbit HRP | 1:2000 | Cell Signaling Technology, Inc., Danvers, MA, USA |
| TLR4 | Mouse monoclonal | 1:500 | Novus Biological, Littleton, CO, USA | Goat anti-rabbit HRP | 1:2000 | Cell Signaling Technology, Inc., Danvers, MA, USA |
| TNFα | Rabbit polyclonal | 1:1000 | Abcam, Cambridge, MA, USA | Goat anti-rabbit HRP | 1:2000 | Cell Signaling Technology, Inc., Danvers, MA, USA |
| TFAM | Rabbit polyclonal | 1:2000 | Abcam, Cambridge, MA, USA | Goat anti-rabbit HRP | 1:2000 | Cell Signaling Technology, Inc., Danvers, MA, USA |
| PGC1α | Rabbit polyclonal | 1:1000 | Novus Biological, Littleton, CO, USA | Goat anti-rabbit HRP | 1:2000 | Cell Signaling Technology, Inc., Danvers, MA, USA |
| FIS1 | Rabbit polyclonal | 1:1000 | Protein Tech Group, Inc., Chicago, IL, USA | Goat anti-rabbit HRP | FIS1 | Cell Signaling Technology, Inc., Danvers, MA, USA |
| MFN2 | Rabbit polyclonal | 1:1000 | Cell Signaling Technology, Inc., Danvers, MA, USA |
Goat anti-rabbit HRP | 1:2000 | Cell Signaling Technology, Inc., Danvers, MA, USA |
| Complex III | Rabbit monoclonal | 1:1000 | Cell Signaling Technology, Inc., Danvers, MA, USA |
Goat anti-rabbit HRP | 1:2000 | Cell Signaling Technology, Inc., Danvers, MA, USA |
| β-actin | Mouse monoclonal | 1:2000 | Millipore Sigma, Burlington, MA, USA | Horse anti-mouse HRP | 1:2000 | Cell Signaling Technology, Inc., Danvers, MA, USA |
Abbreviations: GFAP, glial fibrillary acidic protein; IBA1, ionized calcium binding adaptor molecule 1; TLR4, toll-like receptor 4; TNFα, tumor necrosis factor-α; TFAM, mitochondrial transcription factor A; PGC1α, peroxisome proliferative activated receptor alpha; FIS1, fission 1; MFN2, mitofusin 2.