FIG. 3.

DNA synthesis on ssDNA and SV40 DNA replication by chimerical DNA pol-prim. (A) Mutant (bars 3 to 11) and wild-type (bars 2 and 12) pol-prim complexes were used to synthesize nucleic acids on ssDNA in the presence of NTPs, dNTPs, and Mg²⁺ for 30 (shaded bars) and 60 (solid bars) min. (B) These enzyme complexes were also used in an SV40 DNA replication system by using mouse cell extracts depleted of endogenous pol-prim. Bars 3 to 11, mutant pol-prim with human p180, p68, p58, and mutant p48; bars 2 and 12, murine (MMMM) and human (HHHH) pol-prim. Bars 1, negative control without pol-prim. The assays were repeated twice, and the mean values are presented. The error bars indicate standard deviations.