FIG. 4.

Interaction of PyV T antigen with recombinant DNA pol-prim. Pol-prim and PyV Tag physically interact (6, 31). BSA (1 μg; bar 0) or purified, recombinant pol-prim complexes (1 μg; bars 1–8) as indicated were immobilized. After incubation with 1 μg of PyV Tag for 1 h at room temperature, Tag–pol-prim complexes were determined with Tag-specific antibody F5 and a mouse-specific peroxidase-coupled secondary antibody. The mean values and standard deviations of three experiments are presented in optical density (OD) units.