TABLE 1.
Enzyme activities of recombinant DNA pol-prim complexes
| Enzyme complexa | Protein concn (mg/ml) | Specific DNA polymerase activity (U/mg) | Specific primase activity (U/mg) |
|---|---|---|---|
| MMMM | 0.23 | 3,560 | 260 |
| HHHh(m129–320) | 0.45 | 3,130 | 170 |
| HHHh(m229–320) | 0.47 | 2,610 | 210 |
| HHHh(m273–320) | 0.34 | 3,750 | 380 |
| HHHh(m257–320) | 1.18 | 2,420 | 500 |
| HHHh(m229–288) | 1.40 | 2,120 | 370 |
| HHHh(m257–288) | 1.42 | 2,390 | 240 |
| HHHh-L266P | 1.37 | 1,970 | 160 |
| HHHh-S262F | 0.72 | 2,870 | 250 |
| HHHh-S262F/L266P | 1.13 | 2,900 | 230 |
| HHHH | 0.13 | 19,690 | 840 |
a
MMMM, murine pol-prim; HHHH, human pol-prim; HHHx, hybrid pol-prim containing mutant p48 subunit plus human subunits p180, p68, and p58.