Figure 3.

CBD-X extract inhibits the migration of neutrophils induced by IL-8. CBD-X-treated neutrophils were seeded onto the 3 µm pore polyester membrane in the upper chamber of a 24-well Boyden chamber. Human IL-8 in a concentration of 1 ng/mL was placed in the lower chamber. After three hours, cells that had migrated through the pores into the lower chamber were collected and subjected to flow cytometry analysis. (a) Representative results for migrated neutrophils toward IL-8 after treatment with (1 and 2 µg/mL) CBD-X. The colors in the dot plots represent the quantity of the cells. The lightest color (i.e., blue) would represent the low number of cells. In contrast, green, yellow and red would represent increasing values, with red indicating the highest values. (b) The number of migrated neutrophils. The means were calculated from healthy donors (black big dot) and each dot represents one case. Data were analyzed in comparison to the “IL-8, DMSO” group and analyzed via One-way ANOVA (Fisher’s LSD test with values p < 0.05 considered statistically significant, (* p < 0.05, ** p < 0.01).