Figure 5.

CBD-X extract inhibits migration of leukocytes to OVA-induced asthma lungs. Mice were induced with OVA-induced asthma and were treated with 150 mg/kg of CBD-X extract. Then, mice were sacrificed and lung fluids were collected. (a) Cells in the lung fluids were stained with anti-mouse APC-CD45, anti-mouse FITC- F480, anti-mouse BV786-LY6G, and PE- Siglec F for flow cytometry analysis. Numbers of leukocytes, eosinophils, macrophages, and neutrophils were calculated in the three different treatments; PBS- vehicle (Black bars), OVA- vehicle (Red bars) and OVA- CBD-X (green bars) (a). The gating strategy is (b) FSC vs. SSC, (c) APC-CD45 vs. SSC, and (d) BV786-LY6G vs. FITC-F4/80. (e) PE- Siglec F vs. SSC out of the negative population gate in (d). The colors in the dot plots represent the quantity of the cells. The lightest color (i.e., blue) would represent the low number of cells. In contrast, green, yellow and red would represent increasing values, with red indicating the highest values. Averages and standard deviations were calculated as the means of two biologically independent experiments, each experiment included five mice per group. Data were analyzed via mixed-effects model (Fisher’s LSD test with values p < 0.05 considered statistically significant, (*** p < 0.001).