Figure 6.

CBD-X treatment in a mouse model of OVA-induced asthma. For sensitization, BALB/c mice received an i.p injection of OVA (100 µg) with 3 mg/mL Inject (Alum) (aluminum hydroxide (40 mg/mL) and magnesium hydroxide (40 mg/mL) on days 1, 7, and 14. PBS was injected as a control. The mice were challenged with an (intranasal) i.n injection of OVA (150 µg) or PBS on days 21, 23, and 26. Mice were injected intraperitoneally with 150 mg/kg CBD-X extract or vehicle as a control on days 20, 21, 23, and 26. After 3 h of the last challenge, mice were euthanized and lung fluids were collected using PBS through lavage.