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. 2024 Oct 10;13(10):884. doi: 10.3390/pathogens13100884

Figure 3.

Figure 3

Depth of coverage for sequencing reads aligned to reference sequences. Cleaned sequence reads were aligned to reference sequences with bowtie2 to visualize the breadth (horizontal) and depth (vertical) of coverage for inferring replicon presence/absence. (a) pXO1 from B. anthracis Ames Ancestor, pBCXO1 from G9241, and pBCXO1 from 03BB87. Only G9898 (bottom row) indicates the presence of a complete pXO1 homologous plasmid. (b) pXO2 from B. anthracis Ames Ancestor. The contemporary B. nitratireducens (3001787537, 3001927717, and 3002861066), contemporary B. paranthracis (3003598852 and 3004184063), archival B. cereus genome B0818, and archival B. paranthracis genome D7434 did not indicate the presence of pXO2 despite receiving annotations for the PGA capsule operon genes. (c) pBC218 from G9241. In the G9241 welder’s anthrax BCG genome, the bps exopolysaccharide capsule genes are housed on the pBC218 plasmid. Again, G9898 was the only genome showing evidence of containing a pBC218-like plasmid.