Table 1.
Isolates with annotations for pXO1 and pXO2 anthrax-associated virulence genes. Entries in the “Gene Length (bp)” column are bolded for annotations that were truncated compared to the reference sequence gene length.
| Isolate Name | Isolation Source | Isolation Location (Country: State) | Isolation Year | Closest Species Type Strain | Gene | Amino Acid Identity (%) | Gene Length (bp) | Reference Gene Length (bp) | |
|---|---|---|---|---|---|---|---|---|---|
| Archival | B0818 | Pustule | USA: NM | 1968 | B. cereus | pagA * | 32.35 | 1628 | 2294 |
| capB_1 | 80.33 | 389 | 1395 | ||||||
| capB_2 | 73.81 | 623 | 1395 | ||||||
| capE | 52.4 | 140 | 143 | ||||||
| B4510 | Ulcer | USA: MA | 1970 | B. pretiosus | pagA | 34.71 | 380 | 2294 | |
| D7434 | Blood | USA: NY | 1976 | B. paranthracis | capB | 90.52 | 1130 | 1395 | |
| capC | 84.5 | 449 | 449 | ||||||
| capA | 60.26 | 1199 | 1235 | ||||||
| capD | 57.17 | 1615 | 1587 | ||||||
| capE | 57.14 | 143 | 143 | ||||||
| E5429 | Blood | USA: CO | 1979 | B. mycoides | pagA | 34.71 | 380 | 2294 | |
| G9898 | Sputum | USA: LA | 1996 | B. tropicus † | pagA_1 | 100 | 2294 | 2294 | |
| pagA_2 | 61.31 | 2282 | 2294 | ||||||
| lef | 100 | 2429 | 2429 | ||||||
| cya | 100 | 2402 | 2402 | ||||||
| Contemporary | 3001787537 | sputum | USA: IL | 2019 | B. nitratireducens | capB | 89.88 | 1181 | 1395 |
| capC | 91.95 | 449 | 449 | ||||||
| capA | 87.08 | 1211 | 1235 | ||||||
| capD | 82.52 | 1586 | 1587 | ||||||
| capE | 82.6 | 143 | 143 | ||||||
| 3001927717 | urine | USA: ND | 2020 | B. nitratireducens | capB | 89.88 | 1181 | 1395 | |
| capC | 91.95 | 449 | 449 | ||||||
| capA | 87.08 | 1211 | 1235 | ||||||
| capD | 82.52 | 1586 | 1587 | ||||||
| capE | 82.6 | 143 | 143 | ||||||
| 3002861066 | urine | USA: CA | 2022 | B. nitratireducens | capB | 89.88 | 1190 | 1395 | |
| capC | 91.95 | 449 | 449 | ||||||
| capA | 87.08 | 1211 | 1235 | ||||||
| capD | 82.52 | 1586 | 1587 | ||||||
| capE | 82.6 | 143 | 143 | ||||||
| 3003598852 | blood | USA: TN | 2022 | B. paranthracis | capB | 90.15 | 1130 | 1395 | |
| capC | 92.62 | 449 | 449 | ||||||
| capA | 87.86 | 1255 | 1235 | ||||||
| capD_1 | 79.69 | 1043 | 1587 | ||||||
| capD_2 | 83.3 | 431 | 1587 | ||||||
| capE | 84.78 | 143 | 143 | ||||||
| 3004184083 | blood | USA: AR | 2023 | B. paranthracis | capB | 90.15 | 1130 | 1395 | |
| capC | 91.95 | 449 | 449 | ||||||
| capA | 87.86 | 1255 | 1235 | ||||||
| capD_1 | 79.69 | 1043 | 1587 | ||||||
| capD_2 | 83.3 | 431 | 1587 | ||||||
| capE | 84.78 | 143 | 143 |
* The pagA annotation for B0818 was likely spurious; † G9898 was noted as a potential new species by TYGS due to its low dDDH values with all BCG type strains. However, G9898 had the highest similarity by ANI and dDDH values to the B. tropicus type strain.