TABLE 2.
Nucleotide(s) added by the TNTase activity of HΔ21 are affected by template sequence
| Template | Sequencea (5′ to 3′) | Quantification of TNTase activityb
|
|||
|---|---|---|---|---|---|
| Expt 1
|
Expt 2 (ratio, CMP/ AMP) | ||||
| CMP | AMP | Ratio, CMP/ AMP | |||
| 14-1 | AAAUCCUCUGAUAC | 8,915 | 2,972 | 3.0 | 4.2 |
| 14-2 | AACCUUACUGAUAC | 10,309 | 5,102 | 2.0 | 2.8 |
| 14-3 | CCAUUCUCUGAUAC | 10,410 | 3,097 | 4.4 | 3.5 |
| 14-4 | GCAUUCUCUGAUAC | 10,465 | 2,377 | 3.4 | |
| 14-5 | UUAUUCUCUGAUAC | 8,127 | 4,702 | 1.7 | |
| 14-6 | AUAUUCUCUGAUAC | 8,444 | 2,390 | 3.5 | |
| 14-14 | AAAGCCUCUGAUAC | 9,127 | 3,661 | 2.5 | |
| 14-15 | AAACGCUCUGAUAC | 9,805 | 2,995 | 3.3 | |
| 14-16 | AAAUCCUCUGAUAU | 8,919 | 5,223 | 1.7 | |
| 14-17 | AAAUCCUCUGAUAA | 1,216 | 989 | 1.2 | 1.2 |
| 14-18 | AAAUCCUCUGAUAG | 140 | 6,087 | 0.0 | 0.0 |
| 14-19 | AAAUCCUCUGAUUC | 1,986 | 5,734 | 0.4 | 0.1 |
| 14-20 | AAAUCCUCUGAAAC | 8,927 | 5,988 | 1.5 | |
| 14-21 | AAAUCCUCUGUUAC | 6,332 | 8,253 | 0.9 | 0.5 |
| 14-22 | AAAUCCUCUCAUAC | 91 | 1,154 | 0.1 | 0.3 |
a
Nucleotides that are different from those in 14-1 are in boldface type.
b
The values in the CMP and AMP columns were quantified by a PhosphorImager and used to calculate the CMP/AMP ratio in experiment 1. The results in experiment 2 were from an independent set of assays. Blanks in this column indicate that the templates were not assayed.