Immune correlates of the TMV-HA-con vaccines. BALB/c mice were divided into two experiments: H1-HA and H3-HA groups. All vaccinated groups were intramuscularly (IM) prime/boost immunized with different vaccines at 0- and 21-days post-vaccination (DPV). Following vaccination, five mice from each group were sacrificed at 21 and 35 DPV for collections of blood, spleens, and lungs. Blood was collected and sera were separated and used for hemagglutination inhibition (HI), microneutralization assays, and ELISA. Splenocytes were isolated for use in ELISpot assays. Lungs were homogenized, and lung supernatants were collected and used in HA-specific ELISA.