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. 2024 Oct 17;16(10):447. doi: 10.3390/toxins16100447

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Size fractionation chromatograms of cnidarian venom preparations using size exclusion column high-performance liquid chromatography (SEC-HPLC). Major peaks are labeled: Peak I, molecular weight (MW) of ~250 kDa to 60 kDa comprising large proteins; Peak II MW ~50 kDa to 30 kDa comprising proteins including porins; Peak III MW ~15 kDa to 5 kDa comprising peptides and molecules investigated in this study; Peak IV MW ~3 kDa to 1 kDa. (A) P. physalis venom; (B) C. fleckeri venom; (C) A. alata venom. The fractions highlighted in Peak III were analyzed as subfractions (Peak III A–D) as well as pooled together.