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Representative chromatograms for unbound lenalidomide in post-ultrafiltration plasma using (A) double blank sample, (B) blank sample spiked with lenalidomide-d5 as internal standard (IS, 1000 ng/mL), (C) blank sample spiked with lenalidomide (upper limit of quantification, 1000 ng/mL), (D) blank sample spiked with lenalidomide (lower limit of quantification, 5 ng/mL) and lenalidomide-d5 (IS, 1000 ng/mL). Panels on the left side are for lenalidomide, and those on the right side for IS.
