Table 2.
Within- and between-run precision and accuracy for quantification of total and unbound lenalidomide in human plasma.
| Nominal Concentration (ng/mL) |
Total Lenalidomide | Unbound Lenalidomide | ||||
|---|---|---|---|---|---|---|
| Predicted Concentration (Mean ± SD) (ng/mL) |
Precision (CV, %) a |
Accuracy (%) b |
Predicted Concentration (Mean ± SD) (ng/mL) |
Precision (CV, %) a |
Accuracy (%) b |
|
| Within-run accuracy and precision (n = 5 sample replicates) | ||||||
| 5 | 5.29 ± 0.17 | 3.29 | 105.86 | 4.61 ± 0.19 | 4.06 | 92.14 |
| 15 | 15.20 ± 0.31 | 2.04 | 101.36 | 14.39 ± 0.72 | 5.01 | 95.91 |
| 300 | 293.75 ± 1.89 | 0.65 | 97.92 | 298.26 ± 5.51 | 1.85 | 99.42 |
| 800 | 744.01 + 12.09 | 1.62 | 93.00 | 776.20 ± 5.63 | 0.73 | 97.02 |
| Between-run accuracy and precision (n = 3 runs with 5 replicates/run) | ||||||
| 5 | 5.06 ± 0.39 | 7.65 | 101.10 | 4.92 ± 0.52 | 10.55 | 98.48 |
| 15 | 14.97 ± 0.48 | 3.22 | 99.80 | 14.09 ± 0.82 | 5.80 | 93.95 |
| 300 | 297.42 ± 5.04 | 1.70 | 99.14 | 292.15 ± 6.59 | 2.26 | 97.38 |
| 800 | 755.59 ± 15.45 | 2.05 | 94.45 | 763.95 ± 15.15 | 1.98 | 95.49 |
a CV (%) = (standard deviation of the predicted concentrations/nominal concentration) × 100. b Accuracy (%) = (predicted concentration/nominal concentration) × 100.