Table 3.
Extraction recovery and matrix effect of the total and unbound fraction of lenalidomide and lenalidomide-d5 (internal standard, IS) in human plasma (n = 6 different human blank plasma).
| Nominal Concentration (ng/mL) |
Extraction Recovery (Mean ± SD, %) | Matrix Effect (Mean ± SD, %) | ||
|---|---|---|---|---|
| Total | Unbound Fraction | Total | Unbound Fraction | |
| Lenalidomide | ||||
| 15 | 49.35 ± 1.65 | 45.26 ± 1.18 | 106.82 ± 3.08 | 100.04 ± 1.50 |
| 300 | 55.23 ± 0.86 | 42.83 ± 0.84 | 103.15 ± 0.67 | 98.16 ± 3.39 |
| 800 | 58.43 ± 0.95 | 46.75 ± 0.66 | 99.69 ± 0.89 | 95.81 ± 0.92 |
| Lenalidomide-d5 (internal standard, IS) | ||||
| 1000 | 35.48 ± 1.16 | 27.73 ± 1.06 | 99.15 ± 2.72 | 97.33 ± 1.94 |