Table 1.
Polymorphism rate for the alfalfa genotypes using ISSR primers.
| No. Crt. | Primer | Primer Sequence |
Band Size (pb) | Total Bands (n) | Polymorphic Bands (np) | Pb(%) | PIC | MI | Rp |
|---|---|---|---|---|---|---|---|---|---|
| 1 | A12 | (GA)6CC | 220–1650 | 25 | 20 | 80.00 | 0.20 ± 0.13 | 3.23 ± 0.18 | 7.53 |
| 2 | A13 | (GT)6CC | 150–1510 | 27 | 19 | 70.37 | 0.24 ± 0.09 | 3.24 ± 0.17 | 7.66 |
| 3 | A17 | (GTG)3GC | 240–2090 | 31 | 31 | 100.00 | 0.30 ± 0.07 | 9.17 ± 0.10 | 16.80 |
| 4 | A21 | (CA)6AC | 280–1320 | 15 | 9 | 60.00 | 0.26 ± 0.11 | 1.41 ± 0.20 | 4.46 |
| 5 | UBC818 | (CA)7G | 300–2120 | 19 | 16 | 84.21 | 0.29 ± 0.09 | 3.94 ± 0.18 | 8.73 |
| 6 | Mean | ISSR primers | 150–2120 | 23.40 | 19.00 | 78.91 | 0.25 ± 0.04 | 4.19 ± 2.93 | 9.03 |
Note: n: Total number of bands; np: Number of polymorphic bands; Pb%: polymorphic bands percentage; PIC: Polymorphic Information Content; MI: Marker Index (MI = EMR ∗ PIC), EMR = np (np/n); Rp (resolving power) = i, where np represents the number of polymorphic locus and Ibi is the band index for each band.