FIG. 6.

Induction of spindle morphology in primary endothelial cells by ORF74. dMVECs (passages 4 and 5) were infected with the retroviral expression construct MIGR1-ORF74 (B and D) or the control vector MIGR (A and C). A reporter gene for GFP is expressed on the same mRNA as ORF74 and translated from an IRES in the retroviral vector (see Materials and Methods). Fluorescence was detected using a 395-nm excitation light source for illumination as seen in panels C and D. Photographs were taken by phase-contrast microscopy at 20× magnification at 10 days postinfection. The whitish hue on cells in panels C and D represents GFP expression. Panels A and B (4× magnification) show the morphologic changes in the culture population as a whole (infection efficiency was ca. 80%).