FIG. 7.

Inhibition of NF-κB activation by ORF74 by DN mutants. KSIMM cells (3 × 10⁵ cells) were transfected with pSG5-ORF74, the NF-κB luciferase reporter plasmid, and increasing amounts of the indicated DN mutant expression constructs. Total DNA inputs were equalized with control vector. Cells were lysed and luciferase expression was quantified at 18 h posttransfection. KSIMM cells were transfected with ORF74 and increasing amounts of DN constructs of IKKα, IKKβ, and IκB. All samples were analyzed in triplicate, and the values were averaged. The transfection efficiencies were ca. 30%.