TABLE 1.

Cytokine and growth factor expression in KSIMM and HUVECs^a

Cytokine	Cell type	Mean ELISA reading (pg/ml) ± SD		Fold increase
		ORF 74	Control
IL-6	HUVECs	223 ± 16	149 ± 6.55	1.5
	KSIMM	1,187 ± 17.6	415 ± 45.9	2.86
IL-8	HUVECs	198 ± 47	121 ± 17	1.6
	KSIMM	86,700 ± 1,110	54,400 ± 298	1.59
RANTES	HUVECs	784 ± 14	56 ± 14	14
	KSIMM	401 ± 59	46 ± 9.9	8.7
GM-CSF	KSIMM	199 ± 22.7	117 ± 2.516	1.7
VEGF	HUVECs	350 ± 30	170 ± 14	2.05
	KSIMM	728 ± 50	596 ± 30	1.22

^aKSIMM cells were transfected with ORF74-pSG5 or pSG5 control vector. Supernatants were collected at 24 h posttransfection. HUVECs (passages 4 and 5) were infected with a retroviral expression vector containing ORF74 (MIGR1-ORF74) or the control vector MIGR. Supernatants were collected at 72 h postinfection. Cell counts were determined to ensure that equal cell numbers per well were sampled. Three wells were sampled for each reading. ELISA data were obtained as described in Materials and Methods. ELISA readings are expressed in picograms per milliliter.