Figure 3.

Neuroprotective property of γ-, δ- and ε-scoliidines on mitochondrial metabolism of the PC12 cell line against H₂O₂-induced oxidative stress. (A) The experiment involved treating cells (5 × 10³ cells per well in a 96-well plate) with γ, δ, and ε scoliidines (1 μmol·L⁻¹) for 4 h at 37 °C. After that, the medium was changed with a solution containing peptide (1 μmol·L⁻¹) and H₂O₂ (0.5 mmol·L⁻¹), and the cells were incubated for an additional 20 h. (B) Protective effects of peptides against neurotoxicity caused by oxidative stress. The data from three separate experiments, each conducted six times, were presented in box-and-whisker plots as percentages relative to the control. A one-way ANOVA was followed by Dunnett’s post-test for statistical analyses. * p < 0.05 for differences between the control [C] and experimental groups, and # in relation to the H₂O₂ group. C (+) represents cells treated with acrylamide at 100 mmol·L⁻¹.