Table 2.

Venom diversity comparison of five studies, including the dataset presented here, on N. nivea venom proteomes. Toxin families: three-finger toxins (3FTx), cytotoxins (CTx), neurotoxins (NTx), non-conventional 3FTx (NC), muscarinic toxins (MTx), snake venom metalloproteinases (svMP), snake venom serine proteases (svSP), aminopeptidases (AP), cysteine-rich secretory protein (CRISP), l-amino acid oxidase (LAAO), 5′-nucleotidase (5N), phosphodiesterase (PDE), carboxylesterase (CE), phospholipase A₂ (PLA₂), phospholipase B (PLB), nerve growth factor (NGF), Kunitz-type inhibitor (KUN), vascular endothelial growth factors (VEGF), hyaluronidases (HYAL), cystatin (CYS), C-type lectins and lectin-related proteins (CTL), and disintegrins (DI). Abbr.: mass spectrometry (MS), area under the curve of the extracted ion chromatograms (AUC XIC), label-free quantification (LFQ), mean spectral intensity (MSI), and normalized spectral abundance factor (NASF).

Toxin Family and Subgroups	This Study	Kazandjian et al., 2021	Nguyen et al., 2022	Tan et al., 2022	McFarlane et al., 2024
3FTx	✓	✓	✓	✓	✓
-CTx	✓	✓	✓	✓	✓
-Short NTx	✓	✓	✓	✓	✓
-Long NTx	✓	✓	✓	✓	✓
-NC NTx	✓	✓	✓	✓	✓
-MTx	✓		✓
-Other 3FTx subgroups			✓
svMP	✓		✓	✓	✓
svSP	✓		✓		✓
AP and other peptidases	✓		✓
Venom Complement C3	✓		✓	✓	✓
CRISP	✓	✓	✓	✓	✓
LAAO	✓		✓	✓	✓
5N	✓		✓	✓	✓
PDE	✓		✓	✓	✓
CE and other esterases	✓		✓	✓	✓
PLA2	✓	✓	✓		✓
PLB	✓		✓
Endonuclease	✓
NGF	✓	✓	✓	✓	✓
KUN	✓	✓	✓	✓	✓
Vespryn	✓		✓	✓	✓
VEGF	✓		✓
HYAL			✓		✓
CYS			✓
CTL			✓
DI			✓
Other proteins and peptides			✓		✓
Proteomics workflow	Shotgun	Top-down	Shotgun	Bottom-up	Bottom-up
(Semi)-quantification	No. of toxins	MS (AUC XIC)	MS (LFQ)	RP-HPLC + MS (MSI)	MS (NASF)