Figure 1.

T cell infiltration profiles in response to injection of autogenous Salmonella-killed vaccines (SV1 and SV2), S. Enteritidis lipopolysaccharide (SE-LPS), or vehicle into the pulp of growing feathers. Twenty-four growing feathers (GF) of 14 to 15 wk old Light-brown Leghorn (LBL) pullets from (A) Trial 2-PHL and (B) Trial 3-Farm were injected with 10 µL of SV1, SV2, SE-LPS, or vehicle (water-oil-water emulsion). Injected GFs from each chicken were collected before injection (0 h) and at 6, 24, 48, and 72 h post-GF injection for leukocyte population analysis. Pulp cell suspensions were prepared from each GF, immunofluorescently stained with fluorescence-conjugated mouse monoclonal antibody (Southern Biotech) to chicken CD3 (T cells), and the percentage of CD3+ pulp cells was determined by fluorescence-based flow cytometry. Data shown are mean ± SEM. For each Trial, n = 4 pullets for SV1, SV2, and LPS, and n = 3 for the vehicle. Due to interactions involving the Trial, two-way ANOVA was conducted for each Trial. Student t-test multiple means comparisons were conducted to identify Treatment (Trt) and Time (h) differences. a–c: for each time point, treatment means without a common letter are different (p < 0.05); w–z: for each treatment, means at each time-point without a common letter are different (p < 0.05).