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. 2024 Oct 11;12(10):1159. doi: 10.3390/vaccines12101159

Figure 4.

Figure 4

Salmonella Enteritidis specific IgG levels in plasma after injection of autogenous Salmonella-killed vaccines (SV1 and SV2), S. Enteritidis lipopolysaccharide (SE-LPS), or vehicle into the pulp of growing feathers. SE-specific IgG levels in the plasma of 14–15 wk old Light-brown Leghorn (LBL) pullets were measured after intradermal injection of SV1, SV2, SE-LPS, or vehicle (water-oil-water emulsion) into the pulp of growing feathers. Data shown were pooled across three Trials. Heparinized blood (1.5 mL) was collected before (0 d) and at 3, 5, 7, 10, 14, 21, and 28 d post-pulp injection. Data are means ± SEM; n = 12 pullets for SV1, SV2, and SE-LPS and n = 6 for vehicle (V). Two-way repeated measures ANOVA; Student t-test multiple means comparison-test was used to determine Treatment (Trt) and Time (Day) differences. a,b: for each time point, treatment means without a common letter are different (p < 0.05); w–z: for each treatment, time means without a common letter are different (p < 0.05). Note: the total SV1 and SV2 immunization doses for Trial 1 were 0.140 mL/bird, and for Trial 2 & 3, 0.165 mL/bird. The total SE-LPS dose was 5 µg/bird for all Trials.