FIG. 2.

Taxol treatment increases the amount of VP22 detected in the cytoplasm of synchronously infected cells. Vero cells were synchronously infected with HSV-1(F) or mock infected, and taxol (20 μg/ml) was added to cells at 4 hpi (3 h post-temperature shift). Cells were stained with antibodies to α-tubulin and VP22 and fixed for indirect immunofluorescence at the times indicated as described in Materials and Methods: α-tubulin staining shown in panels A, D, G, J, and M; VP22 shown in panels B, E, H, K, and N; merged images (overlay) shown in panels C, F, I, L, and O. All images were acquired under the same conditions.